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Image Search Results
Journal: Frontiers in Immunology
Article Title: The Microenvironment in Barrett’s Esophagus Tissue Is Characterized by High FOXP3 and RALDH2 Levels
doi: 10.3389/fimmu.2018.01375
Figure Lengend Snippet: Antibodies for FACS staining.
Article Snippet:
Techniques: Staining
Journal: Frontiers in Immunology
Article Title: The Microenvironment in Barrett’s Esophagus Tissue Is Characterized by High FOXP3 and RALDH2 Levels
doi: 10.3389/fimmu.2018.01375
Figure Lengend Snippet: Lower expression of CD1a in Barrett’s esophagus (BE) is associated with lower numbers of CD3 + CD8 + -cells in tissue. Panel (A) depicts expression of CD1a by RT-PCR performed on a total of: reflux esophagitis (RE) biopsies from inflamed squamous tissue (INFL RE, n = 9), non-inflamed squamous esophageal epithelium from RE patients (SQ RE, n = 9), squamous esophageal epithelium from controls (SQ C, n = 12), squamous esophageal epithelium from BE patients (SQ BE, n = 10), BE biopsies (BE, n = 11), duodenal biopsies from BE patients (DUO BE, n = 10), and duodenal biopsies from controls (DUO C, n = 5). CD1a expression was corrected for GAPDH, 2 −ΔCT ± SEM. Panel (B) represents the percentage of CD3 + CD8 + -cells from ex vivo cultures of 7 biopsies from inflamed RE tissue (INFL RE), 6 non-inflamed squamous esophageal biopsies from RE patients (SQ RE), 39 controls (SQ C), 17 squamous esophageal biopsies from BE patients (SQ BE), 19 BE biopsies (BE), 15 duodenal biopsies from BE patients (DUO BE), and 11 duodenal biopsies from controls (DUO C). Each bar represents the mean value ± SEM of the percentage of CD3 + CD8 + -cells in the CD3 + population, determined by flow cytometry. Data were analyzed using the Kruskal–Wallis test (CD1a: p < 0.0001, CD3 + CD8 + : p = 0.0002). p -values from comparing individual groups were obtained by using Mann–Whitney U test in case of individual groups, and Wilcoxon signed rank test for different tissue types from the same patient. Adjustment for multiple comparison was conducted by Benjamini–Yekutieli method. (C) Dot plot of a CD3 + , CD4 + , and CD8 + staining on lymphocytes from ex vivo culture of duodenal tissue from BE patient. Cells were gated first on a forward sideward scatter plot and for CD3.
Article Snippet:
Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Reflux, Ex Vivo, Flow Cytometry, MANN-WHITNEY, Staining
Journal: Frontiers in Immunology
Article Title: The Microenvironment in Barrett’s Esophagus Tissue Is Characterized by High FOXP3 and RALDH2 Levels
doi: 10.3389/fimmu.2018.01375
Figure Lengend Snippet: Similar CD11c expression in Barrett’s esophagus (BE) and duodenal tissues coincides with higher numbers of CD3 + CD4 + -cells. Panel (A) depicts expression of CD11c by real-time-PCR performed on a total of: biopsies from inflamed tissue in reflux esophagitis (RE) patients (INFL RE, n = 9), non-inflamed squamous esophageal epithelium from RE patients (SQ RE, n = 9), squamous esophageal epithelium from controls (SQ C, n = 12), squamous esophageal epithelium from BE patients (SQ BE, n = 10), BE biopsies (BE, n = 11), duodenal biopsies from BE patients (DUO BE, n = 10), and duodenal biopsies from controls (DUO C, n = 5). CD11c expression was corrected for GAPDH, 2 −ΔCT ± SEM. Panel (B) represent the percentage of CD3 + CD4 + -cells from ex vivo cultures of 7 biopsies from inflamed RE tissue (INFL RE), 6 non-inflamed squamous esophageal biopsies from RE patients (SQ RE), 39 controls (SQ C), 17 squamous esophageal biopsies from BE patients (SQ BE), 19 BE biopsies (BE), 15 duodenal biopsies from BE patients (DUO BE), and 11 duodenal biopsies from controls (DUO C). Each bar represents the mean value ± SEM of the percentage of CD3 + CD4 + -cells in the CD3 + -population, determined by flow cytometry. Data were analyzed using the Kruskal–Wallis test (CD11c: p = 0.05, CD3 + CD4 + : p = 0.0002). p -Values from comparing individual groups were obtained by using Mann–Whitney U test in case of individual groups, and Wilcoxon signed rank test for different tissue types from the same patient. Adjustment for multiple comparison was conducted by Benjamini–Yekutieli method.
Article Snippet:
Techniques: Expressing, Real-time Polymerase Chain Reaction, Reflux, Ex Vivo, Flow Cytometry, MANN-WHITNEY